Investigation of Phytochemical, Chemical Composition and Antimicrobial Activities of Noni Leaf (Morindacitrifolia Linn)

Dr. MiMi Yee

Authors

Dr. MiMi Yee

Keywords:

Morindacitrifolia Linn, Phytochemical, Antimicrobial activities, FT-IR spectroscopy

Abstract

Morindacitrifolia Linn (popularly known as “Noni”) has been used in folk medicine by Polynesians for over 2,000 years. It is reported to have a broad range of therapeutic effects, including effects against headache, fever, arthritis, gingivitis, respiratory disorders, infections, tuberculosis, and diabetes. The aim of this study was to investigate the chemical constituents from leaves of MorindacitrifoliaLinn (Noni). Preliminary phytochemical investigation on leaf of Morindacitrifolia Linn indicated the presence of steroids, terpenoids, flavonoids, glycosides, phenolic compounds, α-amino acids, reducing sugars, carbohydrates, saponins, tannins and alkaloids. The determination of elemental analysis by AAS method suggested that K (111.80 ppm), Ca (103.40 ppm), Fe (7.67 ppm), Mn (4.15 ppm) and Zn (1.15 ppm). By column chromatographic separation technique, isolated compound β-sitosterol (A, 0.029%) and ursolic acid (B, 0.01%) were isolated from PE crude extract of leaf of Morindacitrifolia Linn (Noni). The isolated compounds were identified by FT-IR spectroscopy. In vitro antimicrobial activity of some crude extracts (pet-ether, ethyl acetate, ethanol, and water) of Noni leaf was screened by agar-well diffusion method. H₂O extract of Noni was found to exhibit potent of antimicrobial activity (25mm) against on B. sub, S. sureous, P. seudomonus, B. pumalis, Candida and E. coli.

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