ISOLATION, PHYTOCHEMICAL INVESTIGATION AND IN-VITRO ANTIBACTERIAL ACTIVITY OF ETANOLIC EXTRACT USING FABIANA IMBRICATA L.

Naga Kumari Kattepogu; Ravi Kumar Konda; Sowjanya Jyothi T; Shaik Shakeela; Madhavi Latha J

doi:10.47957/ijciar.v6i1.150

Authors

Kattepogu Naga Kumari Hindu College of Pharmacy, Guntur-522002, Andhra Pradesh, India
Konda Ravi Kumar Hindu College of Pharmacy, Guntur-522002, Andhra Pradesh, India
T.Sowjanya Jyothi Hindu College of Pharmacy, Guntur-522002, Andhra Pradesh, India
Shaik Shakeela Hindu College of Pharmacy, Guntur-522002, Andhra Pradesh, India
J.Madhavi Latha Hindu College of Pharmacy, Guntur-522002, Andhra Pradesh, India

DOI:

https://doi.org/10.47957/ijciar.v6i1.150

Keywords:

Fabiana imbricata, Ciprofloxacin, Anti-antibacterial activity, Escherichia coli, Bacillus Subtilis

Abstract

Fabiana imbricata is a medicinal plant with therapeutic potential, belongs to the family Solanaceae also known as, Made mustard and is known for its anti-bacterial activity. I have been selected groups were divided into positive control, negative control, test groupsT1(20µg/ml)_, T2(40µg/ml)_, T3(60µg/ml)_, T4(80µg/ml) and T5(100µg/ml),standard group ciprofloxacin 2mg/ml and also prepared the stock solutions and the same way prepared the cultured medium by using the formula from the cultured medium taken and prepared sub cultured medium by using microorganisms. The result of antibacterial activity suggested that effects ofFabiana imbricata plant extract were tested on phytochemical, results showed the presence of alkaloids, saponins, Phenolic compounds flavonoids, tannins as chemical constituents may have anti-bacterial activity, which is not evaluated till now.Among that result, shows alkaloids and flavonoids were most effective compounds for the antibacterial activity in higher plants. Apart from that saponins show the most responsible and effectively compound for antibacterial activity of Fabiana imbricata. In this study, two solvents were taken ethanolic whole plant extracts from Fabiana imbricata have been tested for Anti-bacterial activity.Fabiana Imbricata was a traditional medicine. We found strong anti-bacterial activity specifically in the ethanol extracts of Fabiana Imbricata. Plant phenolic compounds have been found to possess potent anti- bacterial activity. These flavonoids have been found to possess anti-bacterial properties in various studies. Strong presence of tannins in all the solvent extracts may explain its potent bioactivities are known to possess potent an anti- bacterial properties. The Saponins have already shown as anti- bacterial activity.

Downloads

Download data is not yet available.

References

Iverson, Cheryl, et al Use of Italics. AMA Manual of Style Oxford, Oxfordshire Oxford University Press. ISBN 978-0-19-517633-9, 2007, 12.1.1, 10th.

American Psychological Association, Use of Italics, The Publication Manual of the American Psychological Association, Washington, DC, USA: APA,ISBN 978-1-4338-0562-2. 2010, 4.21, 6.

Alberts, Bruce Molecular biology of the cell. New York: Garland Science.ISBN 0-8153-4105-9, 2008.

Vignais, Paulette M, Pierre Vignais Discovering Life, and Manufacturing Life How the experimental method shaped life sciences. Berlin Springer, ISBN 90-481-3766-7, 2010. DOI: https://doi.org/10.1007/978-90-481-3767-1

Jacqueline Nairn, Price, Nicholas C,Exploring proteins, a student's guide to experimental skills and methods. Oxford, Oxfordshire, Oxford University Press, ISBN 0-19-920570-1, 2009.

Sunshine, Geoffrey, Coico, Richard Immunology, a short course. Wiley-Blackwell, ISBN 0-470-08158-9, 2009.

Existing Non-animal Alternatives,"AltTox, org, 8 September 2011.

Rothman, S. S,Lessons from the living cell, the culture of science and the limits of reductionism, New York, McGraw-Hill, ISBN 0-07-137820-0 2002.

De Clercq E Recent highlights in the development of new antiviral drugs, Curr,Opin, Microbial, 8, 5, October, 2005. DOI: https://doi.org/10.1016/j.mib.2005.08.010

Ferrero, Miliani L, Nielsen OH, Andersen PS, Girardin SE; Nielsen; Andersen,Girardin 147, 2,061127015327006,doi,PMID 17223962, February 2007. DOI: https://doi.org/10.1111/j.1365-2249.2006.03261.x

bbas A.B, Lichtman A.H In Saunders Elsevier, Basic Immunology, Functions and disorders of the immune system , Medical Dictionary Twenty-fifth ed, Williams & Wilkins,ISBN 978-1-4160-4688-2, 3rd ed, 1990,2009.

Rather, L. J,Disturbance of function functiolaesa the legendary fifth cardinal sign of inflammation, added by Galen to the four cardinal signs of Celsus" Bull N Y Acad Med 47 3,303–322, PMC 1749862.,PMID 5276838,1971.

A Massage Therapist Guide to Pathology Ruth Werner,a massage Therapist Guide to Pathology, Philadelphia, PA and Baltimore, MD, Wolters Kluwer, 4th ed, 2009.

Vogel, Wolfgang H, Berke, Andreas Brief History of Vision and Ocular Medicine, Kugler Publications, 97, ISBN 90-6299-220-X, 2009.

Porth, Carol Essentials of pahtophysiology, concepts of altered health states. Hagerstown, MD: Lippincott Williams & Wilkins, p, 270, ISBN 0-7817-7087-4, 2007.

Dormandy, Thomas,The worst of evils, man's fight against pain, New Haven, Conn, Yale University Press, p, 22.,ISBN 0-300-11322-6,2006.

Wiedermann U et al, "Vitamin A deficiency increases inflammatory responses, Scand J Immunol, 44, 6,578–584, 1996. DOI: https://doi.org/10.1046/j.1365-3083.1996.d01-351.x

Hargrave, B. Y, Tiangco, D. A, Lattanzio, F. A.Beebe, S. J, Cocaine, not morphine, causes the generation of reactive oxygen species and activation of NF-κB in transiently cotransfected heart cells, CardiovascToxicol 141–151, 3, 2, 2003. DOI: https://doi.org/10.1385/CT:3:2:141

Montiel-Duarte, C, Nazarene, E, López-Zabalza, M. J, Cenarruzabeitia, E, Iraburu, M. J. "Role of reactive oxygen species, glutathione and NF-κB in apoptosis induced by 3, 4-methylenedioxymethamphetamine,Ecstasy on hepatic stellate cells, BiochemPharmacol ,1025–1033, doi,10.1016/j.bcp.2003,10.020, PMID 15006539, 67 ,6, 2004. DOI: https://doi.org/10.1016/j.bcp.2003.10.020

HendrikUngefroren, Susanne Sebens, DanieSeidl, HendrikLehnert, Ralf Haas Cell Communication and Signaling,doi,10,1186/1478-811X-9-18,9, 18, 2011. DOI: https://doi.org/10.1186/1478-811X-9-18

Konda RK. Brief description of Clinical Case study formats: a basic review. Journal of Case Studies and Case Reports. 2022 Apr 30:24-6.

Coussens, L. M, Werb, Z, Nature 860–867, doi,10.1038/nature01322. PMC 2803035, PMID 12490959, 420, 6917,2002. DOI: https://doi.org/10.1038/nature01322

Gunn, L, Ding, C; Liu, M; Ma, Y. Qi, C. Cai, Y. Hu, X, Aggarwal, D, Zhang, HG, Yan, J Opposing roles for complement component C5a in tumor progression and the tumor microenvironment, Journal of immunology Baltimore, Md. , 1950, 2985–94, doi,10.4049/jimmunol.1200846. PMID 22914051,189, 6, Sep 15, 2012. DOI: https://doi.org/10.4049/jimmunol.1200846