• Kadhim H. A. Al-Ameri Science department, College of Basic Education, University of Sumer, Iraq
  • Ashjan Agar Nasser Science department, College of Basic Education, University of Sumer, Iraq



Melanin, Staph, aureus, nitrogen source, carbon source, temperature and pH


The current study included isolating Staphylococcus aureus from the urine of men with urinary tract infections, where 17 isolates belonging to this species were obtained, which were diagnosed traditionally and by polymerase chain reaction. Then, the ability of the isolates to produce melanin was tested, as it is a substance that has great medical benefits, as it can be isolated and used as an antibacterial and antifungal, it has many other benefits. All isolates were melanin-producing, but the best of them were isolates 1, 2, 3and 10, which gave melanin amount of 3.1, 2.8, 3, and 2.9 mg/L, respectively, and gave a dry weight of 8.8, 6.5, and 6.1and 3.6 mg/l.      Nitrogen sources gave close amounts of melanin, as follows: soybeans produced melanin with an amount of 2.7, yeast extract 2.6, and peptone 2.65 mg/l, while the best carbon sources culture medium was starch2.8 mg/l, then glucose came after that with 2.6 mg/l, then glycerol, maltose, fructose, sucrose, dextrose, and mannitol, with a yield of 2.4, 2.2, 2.2, 1.7, and 1.5 mg/l, respectively.      The best temperature for melanin production was 35℃, followed by 30℃, then 25℃, then 40℃, then 20℃, with a melanin production amount of, 2.8, 2.3, 1.9, 1.7 and 1.1mg\l respectively. As for the pH, the best was 7, followed by 6, then 8, followed by 5, then 4 with melanin amount of  2.7,2.2, 1.7,1.7 and 1.3 mg\lrespectively.


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P. A. Riley, "Melanin," The international journal of biochemistry & cell biology, vol. 29, no. 11, pp. 1235-1239, 1997. DOI:

K. Jimbow, W. C. Quevedo Jr, T. B. Fitzpatrick, and G. Szabo, "Some aspects of melanin biology: 1950–1975," Journal of Investigative Dermatology, vol. 67, no. 1, pp. 72-89, 1976. DOI:

K. Van Den Bossche, J. M. Naeyaert, and J. Lambert, "The quest for the mechanism of melanin transfer," Traffic, vol. 7, no. 7, pp. 769-778, 2006. DOI:

A. S. ElObeid, A. Kamal‐Eldin, M. A. K. Abdelhalim, and A. M. Haseeb, "Pharmacological properties of melanin and its function in health," Basic & clinical pharmacology & toxicology, vol. 120, no. 6, pp. 515-522, 2017. DOI:

W. M. Bandaranayake, "The nature and role of pigments of marine invertebrates," Natural Product Reports, vol. 23, no. 2, pp. 223-255, 2006. DOI:

J. P. Ortonne, "Photoprotective properties of skin melanin," British Journal of Dermatology, vol. 146, pp. 7-10, 2002. DOI:

S. Singh et al., "Microbial melanin: Recent advances in biosynthesis, extraction, characterization, and applications," Biotechnology Advances, vol. 53, p. 107773, 2021. DOI:

J. D. Nosanchuk and A. Casadevall, "Impact of melanin on microbial virulence and clinical resistance to antimicrobial compounds," Antimicrobial agents and chemotherapy, vol. 50, no. 11, pp. 3519-3528, 2006. DOI:

A. A. H. Mohi and M. K. A. Al-Shibly, "Investigation of Melanin in Dermatophytes that Isolated from Primary Schools Students," Journal of Global Pharma Technology, vol. 11, no. 05, 2009.

W. C. Fuqua and R. M. Weiner, "The melA gene is essential for melanin biosynthesis in the marine bacterium Shewanella colwelliana," Microbiology, vol. 139, no. 5, pp. 1105-1114, 1993. DOI:

G. F. M. Gad, M. A. El-Feky, M. S. El-Rehewy, M. A. Hassan, H. Abolella, and R. M. Abd El-Baky, "Detection of icaA, icaD genes and biofilm production by Staphylococcus aureus and Staphylococcus epidermidis isolated from urinary tract catheterized patients," The Journal of Infection in Developing Countries, vol. 3, no. 05, pp. 342-351, 2009. DOI:

P. M. Leighton and J. A. Little, "Identification of Coagulase-Negative Staphylococci Isolated from Urinary Tract Infections," American Journal of Clinical Pathology, vol. 85, no. 1, pp. 92-95, 1986, doi: 10.1093/ajcp/85.1.92. DOI:

B. Hovelius and P.-A. Mårdh, "Staphylococcus saprophyticus as a common cause of urinary tract infections," Reviews of infectious diseases, vol. 6, no. 3, pp. 328-337, 1984. DOI:

N. K. Priest et al., "From genotype to phenotype: can systems biology be used to predict Staphylococcus aureus virulence?," Nature Reviews Microbiology, vol. 10, no. 11, pp. 791-797, 2012. DOI:

F. Martineau et al., "Correlation between the resistance genotype determined by multiplex PCR assays and the antibiotic susceptibility patterns of Staphylococcus aureus and Staphylococcus epidermidis," Antimicrobial agents and chemotherapy, vol. 44, no. 2, pp. 231-238, 2000. DOI:

R. Ali, K. Al-Achkar, A. Al-Mariri, and M. Safi, "Role of polymerase chain reaction (PCR) in the detection of antibiotic-resistant Staphylococcus aureus," Egyptian Journal of Medical Human Genetics, vol. 15, no. 3, pp. 293-298, 2014. DOI:

A. van Belkum and H. Verbrugh, "40 years of methicillin resistant Staphylococcus aureus: MRSA is here to stay—but it can be controlled," vol. 323, ed: British Medical Journal Publishing Group, 2001, pp. 644-645. DOI:

V. Vasanthabharathi, R. Lakshminarayanan, and S. Jayalakshmi, "Melanin production from marine Streptomyces," African Journal of Biotechnology, vol. 10, no. 54, p. 11224, 2011. DOI:

T. Dwyer, H. K. Muller, L. Blizzard, R. Ashbolt, and G. Phillips, "The use of spectrophotometry to estimate melanin density in Caucasians," Cancer epidemiology, biomarkers & prevention: a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, vol. 7, no. 3, pp. 203-206, 1998. DOI:

K. J. McGraw, "An update on the honesty of melanin‐based color signals in birds," Pigment Cell & Melanoma Research, vol. 21, no. 2, pp. 133-138, 2008. DOI:

D.-X. Tan, L. C. Manchester, R. M. Sainz, J. C. Mayo, J. León, and R. J. Reiter, "Physiological ischemia/reperfusion phenomena and their relation to endogenous melatonin production: an hypothesis," Endocrine, vol. 27, pp. 149-157, 2005. DOI:

T. Foster, "Staphylococcus," Medical Microbiology. 4th edition, 1996.

J. Bien, O. Sokolova, and P. Bozko, "Characterization of virulence factors of Staphylococcus aureus: novel function of known virulence factors that are implicated in activation of airway epithelial proinflammatory response," Journal of pathogens, vol. 2011, 2011. DOI:

Y. Feng, C.-J. Chen, L.-H. Su, S. Hu, J. Yu, and C.-H. Chiu, "Evolution and pathogenesis of Staphylococcus aureus: lessons learned from genotyping and comparative genomics," FEMS microbiology reviews, vol. 32, no. 1, pp. 23-37, 2008. DOI:

E. Huntzinger et al., "Staphylococcus aureus RNAIII and the endoribonuclease III coordinately regulate spa gene expression," The EMBO journal, vol. 24, no. 4, pp. 824-835, 2005. DOI:

M. I. Halpin-Dohnalek and E. H. Marth, "Staphylococcus aureus: Production of extracellular compounds and behavior in foods-A review," Journal of food protection, vol. 52, no. 4, pp. 267-282, 1989. DOI:

C. Tassou, K. Koutsoumanis, and G.-J. Nychas, "Inhibition of Salmonella enteritidis and Staphylococcus aureus in nutrient broth by mint essential oil," Food research international, vol. 33, no. 3-4, pp. 273-280, 2000. DOI:

R. A. Mah, D. Y. Fung, and S. A. Morse, "Nutritional requirements of Staphylococcus aureus S-6," Applied microbiology, vol. 15, no. 4, pp. 866-870, 1967. DOI:

G. Gladstone, "The nutrition of Staphylococcus aureus; nitrogen requirements," British Journal of Experimental Pathology, vol. 18, no. 4, p. 322, 1937.

J. Sutherland, A. Bayliss, and T. Roberts, "Predictive modelling of growth of Staphylococcus aureus: the effects of temperature, pH and sodium chloride," International journal of food microbiology, vol. 21, no. 3, pp. 217-236, 1994. DOI:

Talib Jawad, S., F Shihab, A., & Al-Taher, Q. M. Heavy metal concentrations in water, sediments, Cladophora and two fish species from Al-Masab Alamm River, Al-Nassiriya, Iraq. Caspian Journal of Environmental Sciences. 2022, 20(4), 805-812.‏

Jawed S T, Al-Taher Q M, Shihab A F. Norouzi S. Melanoides tuberculate as Bioindicator of the Heavy Metal Contamination in Water and Sediment Pollution of Euphrates River at Thi-Qar province, Iraq. Health Education and Health Promotion. 2022 ;10(2):247-253.

Al-Taher, Q. M., Akbar, M. M., & Al-Qarooni, I. H. Estimation of heavy metals in water, sediments and bioaccumulation in pseudodontopsis Euphraticus and bellamya bengalensis in Euphrates river in al-nassiriyah city/south of Iraq. Plant Archives. 2020, 20(2), 1454-1460.‏

ZADEH, Firoozeh Abolhasani, et al. Cytotoxicity evaluation of environmentally friendly synthesis Copper/Zinc bimetallic nanoparticles on MCF-7 cancer cells. Rendiconti Lincei. Scienze Fisiche e Naturali, 2022, 1-7. DOI:

ROHMAH, Martina Kurnia, et al. Modulatory role of dietary curcumin and resveratrol on growth performance, serum immunity responses, mucus enzymes activity, antioxidant capacity and serum and mucus biochemicals in the common carp, Cyprinus carpio exposed to abamectin. Fish & Shellfish Immunology, 2022, 129: 221-230.‏ DOI:

ARIF, Anam, et al. The functions and molecular mechanisms of Tribbles homolog 3 (TRIB3) implicated in the pathophysiology of cancer. International Immunopharmacology, 2023, 114: 109581.‏ DOI:

MARGIANA, Ria, et al. Functions and therapeutic interventions of non-coding RNAs associated with TLR signaling pathway in atherosclerosis. Cellular Signalling, 2022, 100: 110471.‏ DOI:

H. A. Al-Hchaimi, M. F. Alhamaidah, H. Alkhfaji, M. T. Qasim, A. H. Al-Nussairi and H. S. Abd-Alzahra, "Intraoperative Fluid Management for Major Neurosurgery: Narrative study," 2022 International Symposium on Multidisciplinary Studies and Innovative Technologies (ISMSIT), 2022, pp. 311-314, doi: 10.1109/ISMSIT56059.2022.9932659. DOI:

LEI, Zimeng, et al. Detection of abemaciclib, an anti-breast cancer agent, using a new electrochemical DNA biosensor. Frontiers in Chemistry, 2022, 10.‏ DOI:

BASHAR, Bashar S., et al. Application of novel Fe3O4/Zn-metal organic framework magnetic nanostructures as an antimicrobial agent and magnetic nanocatalyst in the synthesis of heterocyclic compounds. Frontiers in Chemistry, 2022, 10.‏ DOI:



How to Cite

Al-Ameri, K. H. A., & Nasser, A. A. (2023). INVESTIGATION OF THE MELANIN INSTAPHYLOCOCCUS AUREUS ISOLATED FROM PATIENTS WITH URINARY TRACT INFECTIONS. International Journal of Current Innovations in Advanced Research, 6(2), 1–8.



Original Articles