DEVELOPMENT AND VALIDATION OF A GREEN UV -SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF ACOTIAMIDE IN TABLET DOSAGE FORM USING WATER AS A GREEN SOLVENT

Abstract

This study describes the Development And Validation of an eco -friendly UV-Spectrophotometric method for the estimation of acotiamide in tablet formulation in accordance with ICH Q2(R1) guidelines. The main aim of this study was to develop simple, accurate, precise and rapid, cost-effective validation method using water as an eco friendly solvent. Water was used as a green solvent to minimize the use of hazardous organic solvents and support a sustainable analytical approach. The method was validated for linearity, accuracy, precision, specificity, Robustness, Ruggedness LOD,LOQ.An eco-friendly uv spectrophotometric method  used for estimation of acotiamide in tablet formulations. In this method the λmax of acotiamide was determined by scanning the standard solution over the wavelength range of 200-400nm .The standard and sample solutions were prepared and their absorbance was measured using uv spectrophotometer .Acotiamide exhibited maximum absorbance at 217nm. This method obeyed Beer – lambert’s law and exhibits a good linearity over the concentration range of 3-11µg/ml with regression equation A = 0.0574C + 0.0836 And a correlation coefficient R² of 0.9999.The accuracy of method was evaluated by recovery studies the percentage recovery was found in the range of 99.0%-99.2 %and the method demonstrated good precison the %RSD values   were found to be less than 2%. Based on the above results the eco friendly UV spectrophotometric validation method is suitable for routine quality control analysis.  The developed method is suitable for routine quality control analysis and assay determination of acotiamide tablets.