Antimicrobial Activity of Marine Gastropod Purpura bufo from Visakhapatnam, East Coast of India

Darwin Chatla; Joseph Uday Ranjan T; Ramesh Babu K

Authors

Darwin Chatla Department of Zoology, Acharya Nagarjuna University, Guntur, A.P., India
Joseph Uday Ranjan, T Department of Zoology, Acharya Nagarjuna University, Guntur, A.P., India
Ramesh Babu, K Department of Marine Living Resources, Andhra University, Visakhapatnam, A.P., India

Keywords:

Purpura bufo, Antimicrobial activity, Inhibition Zone, Methanol extract

Abstract

The present study was aimed to investigate the antimicrobial activity of marine gastropod i.e. Purpura bufo. The antimicrobial activity was estimated in extracts of body tissues of Purpura bufo against Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, and fungi like Aspergillus flavus and Penicillium notatum. The maximum inhibition zone was observed in Klebsiella pneumonia (26 mm) and minimum in Penicillium notatum (11 mm). It is evident from the current study that significant reduction in growth of bacteria and fungi was observed with methanolic extracts.

Downloads

Download data is not yet available.

References

Anand, T.P. and Patterson Edward, J.K. 2002. Antimicrobial activity in the tissue extracts of five species cowries Cypraea spp. (Mollusca: Gastropoda) and an ascidian Didemnum psammathodes (Tunicata: Didemnidae). Indian J. Mar. Sci., 31(3): 239-242.

Anand, T.P., Rajaganapathy, J. and Edward, J.K.P. 1997. Antibacterial activity of marine mollusks from Portonovo region. Ind. J. Mar. Sci., 26: 206-208.

Avila, C., Iken, K., Fontana, A. and Cimino, G. 2000. Chemical ecology of the Antarctic nudibranch Bathydoris hodgsoni Eliot, 1907: Defensive role and origin of its natural products. Nat. Prod. Lett., 3: 31-35.

Bazes, A., Silkina, A., Douzenel, P., Faÿ, F., Kervarec, N., Morin, D., Berge, J.P. and Bourgougnon, N., 2009. Investigation of the antifouling constituents from the brown alga Sargassum muticum (Yendo) Fensholt. Journal of Applied Phycology, 21(4): 395-403.

Berkholder, P.R. and Burkholder, L.M. 1958. Antimicrobial activity of horny corals. Science., 127: 1174.

Chellaram, C.M.E.G. and Patterson, E.J.K. 2004. Antibacterial activity of the winged oyster Pteria chinensis (Pterioida: Pteridae). Indian J. Mar. Sci., 33: 369- 372.

Darwin, Ch. and Padmavathi, P. 2018. Preliminary assessment of calcium in six molluscan shells of Tamilnadu coast, India. Eco. Env. Cons., 24 (1): 302-305.

Faulkner DJ. Marine natural products. 2002. Nat Prod Rep. 19:1-48.

Fernando, O. and Fernando, J. 2002. A field guide to the common invertebrates of the East coast of India. Annamalai University, India. 1-258.

Haug, T., Stensverg, K., Olsen, O., Sandsdalen, E. and Styrovld, O.B. 2003. Antibacterial activities in various tissue of the horse mussel Modiolus modiolus. Invertebr. Pathol., 85: 112-119.

Indap, M.M. and Pithare, S.P. 1998. Cytotoxicity and bioactivity of some marine animals. Indian. J. Mar. Sci., 27: 433-437.

Karuso P. Chemical ecology of the nudibranches. 1987. Bioorg Mar Chem. 1:31-60.

Kumaran, N., Bragadeeswaran, S. and Meenakshi, V.K. 2011. Evaluation of antibacterial activity of crude extracts of ascidian Didemnum psammathodes Sluiter, 1895 against isolated human and fish pathogens. Asian Pacific J.Trop. Biomed., S90-S99.

Laith, A.A., Ambak, M., Abol-Munafi, A.B., Nurhafizah, W.W.I. and Najiah, M. 2017. Metabolomic analysis of marine and mud crabs based on antibacterial activity. Aquaculture Reports, 7: 7-15.

Mayer, A.M., Rodriguez, A.D., Berlinck, R.G. and Fusetani, N. 2011. Marine pharmacology in 2007-8: Marine compounds with antibacterial, anticoagulant, antifungal, anti-inflammatory, antimalarial, antiprotozoal, antituberculosis, and antiviral activities; affecting the immune and nervous system, and other miscellaneous mechanisms of action. Comp. Biochem. Physiol., 153C: 191-222.

Mayer, A.M.S., Rodriguez, A.D., Berlinck, R.G.S. and Hamman, M.T. 2007. Marine pharmacology in 2003–2004: Marine Compounds with anthelminthic, antibacterial, anticoagulant, antifungal, antiinflammatory, antimalarial, antiplatelet, antiprotozoal, anti–tuberculosis, and antiviral activities; affecting the cardiovascular, immune and nervous systems, and other miscellaneous mechanisms of action. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology., 145: 553–581.

Mitta, G., Hubert, F., Noel, T., Roch, P., Myticin. 1999. A novel cysteine-rich antimicrobial peptide isolated from haemocytes and plasma of the mussel Mytilus galloprovincialis. Eur. J. Biochem., 265: 71–78.

Nakamura, T., Furunaka, H., Miyata, T., Tokunaga, F., Muta, T., Iwanaga, S, et al., 1988. A class of antimicrobial peptide from the hemocytes of the horseshoe crab, (Tachypleus tridentatus). Isolation and chemical structure. J. Biol. Chem., 263: 16709-13713.

Narahashi, T. 1988. In: Marine Toxins and Venoms (edited by Tu, A.T.), Marcel Dekker, Inc., New York, 195 pp.

Olicard, C., Renault, T., Torhy, C., Benmansour, A., Bourgougnon, N. 2005. Putative antiviral activity in hemolymph from adult Pacific oysters, Crassostrea gigas. Antivir. Res., 66: 147–152.

Proksch, P., Edrada, R. and Ebel, R., 2002. Drugs from the seas–current status and microbiological implications. Applied Microbiology and Biotechnology. 59(2-3):125-134.

Rajaganapathi, J. 2001. Antimicrobial activates of marine mollusca purification of anti- HIV protein. Ph.D. Thesis, India.

Roch, P., Yang, Y., Toubiana, M., Aumelas, A. 2008. NMR structure of mussel mytilin, and antiviral–antibacterial activities of derived synthetic peptides. Dev. Comp. Immunol., 32: 227–238.

Santhana Ramasamy, M. and Murugan, A. 2003. Chemical defense in ascidians Eudistoma viride and Didemnum psammathodes in Tuticorin, Southeast coast of India: Bacterial epibiosis and fouling deterrent activity. Indian J. Mar. Sci., 32: 4337-339.

Yoneyama, Hiroshi, and Ryoichi Katsumata. 2006. Antibiotic resistance in bacteria and its future for novel antibiotic development. Biosci. Biotech. Biochem., 5: 1060-1075.

Zasloff, M. 2002. Antimicrobial peptides of multicellular organisms. Nature., 415: 389–395.